IIS 6 suddenly requires authentication on a website

Basically, all I did wa开发者_开发问答s redirect an old website in IIS management to a new local directory, and now all the pages suddenly require the HTTP authentication. I have checked the settings, and Enable Anonymous Access is checked, and lists a guest user. I then tried creating a new local user, making it guest-group member only, and entered that into the website IIS settings to use.

Still no luck. Why does IIS suddenly want authentication? What changed??

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Check the permissions on the directory to ensure that the user Internet Guest Account has read access.

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Check account under which apppool is running. Does it have access on website folder?