Error Type: Microsoft VBScript runtime (0x800A0046) Permission denied

I am getting this error when i try to access an asp website which has been hosted in IIS 5.

Error开发者_如何学C Type: Microsoft VBScript runtime (0x800A0046) Permission denied /Site/Initial.asp, line 164

Please let me know what could be is issue???

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Edit IIS Default Web Site Properties.

In the Directory Security tab, make sure the checkbox for user is checked.